생물학 액틴 세포골격(actin cytoskeleton) 이미징(영문)

생물학 액틴 세포골격(actin cytoskeleton) 이미징(영문) [생물학] 액틴 세포골격(actin cytoskeleton) 이미징(영문).pptx 목차 1.Function of actin 2.Actin imaging 3.Application 4.Summary 본문 Actin polymerization ATP bound actin polymerizes to form polar microfilament Cell morphology, motility Tightly regulated, coordinated by different actin-binding proteins 3. Intracellular transport - Microfilament is concerned with intracellular transport of vesicles or other organelles - Mitochondria transport in cells Phalloidin method used in many actin detection (since 1970) Limitations -less effective in live cell imaging -Toxic effects on cell Limitations Photobleaching Robust chromophores needed Alexa, Seta or DyLight Flours Minimized light exposure Cell membrane permeability Expression of transgenic protein 본문내용 unction of actin Actin imaging Application Summary Actin Cytoskeleton Actin Monomer globular protein with two distinct ends Barbed end Pointed end Binds to ATP or ADP Actin Cytoskeleton Actin polymerization ATP bound actin polymerizes to form polar microfilament Cell morphology, motility Tightly regulated, coordinated by different actin-binding proteins Actin Cytoskeleton Actin Cytoskeleton 참고문헌 http://www.nature.com/nmeth/journal/v5/n7/full/nmeth.1220.html http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005744 http://zeiss-campus.magnet.fsu.edu/galleries/video/csu/videos/bsc1memeraldlifeactmcherrylysosomes/index.html http://zeiss-campus.magnet.fsu.edu/galleries/static/cells/pl1ut2.html